Prokaryotic Nucleotide Excision Repair

Prokaryotic nucleotide excision repair.

Nucleotide excision repair (NER) has allowed bacteria to flourish in many different niches around the globe that inflict harsh environmental damage to their genetic material. NER is remarkable because of its diverse substrate repertoire, which differs greatly in chemical composition and structure. Recent advances in structural biology and single-molecule studies have given great insight into th...

Prokaryotic nucleotide excision repair: the UvrABC system.

Department of Pharmacological Sciences, State University of New York at Stony Brook, Stony Brook, New York 11794-5115, Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, and Rudolf Virchow Center for Experimental Biomedicine, Institute for Structural Biology, University of Würzburg, ...

SnapShot: Nucleotide Excision Repair

Nucleotide Excision Repair and Cancer

Cancer ranks as one of the most frequent causes of death worldwide and in Western society it is competing with cardiovascular disease as the number one killer. This high frequency in Western countries can be attributed to lifestyle and environmental factors, only 5-10% of all cancers are directly due to heredity. Common environmental factors leading to cancer include: tobacco (25-30%), diet and...

Removal of Misincorporated Ribonucleotides from Prokaryotic Genomes: An Unexpected Role for Nucleotide Excision Repair

Stringent steric exclusion mechanisms limit the misincorporation of ribonucleotides by high-fidelity DNA polymerases into genomic DNA. In contrast, low-fidelity Escherichia coli DNA polymerase V (pol V) has relatively poor sugar discrimination and frequently misincorporates ribonucleotides. Substitution of a steric gate tyrosine residue with alanine (umuC_Y11A) reduces sugar selectivity further...